Total Phenolic Content, Total Flavonoid Content and Radical Scavenging Activity from Zingiber zerumbet Rhizome using Subcritical Water Extraction

Authors

hizen Conversion and Separation Technology (Shizen ikohza) Malaysia-Japan International Institute of Technlogy (MJIIT), Universiti Teknologi Malaysia Kuala Lumpur, Jalan Sultan Yahaya Petra, 54100 Kuala Lumpur, Malaysia

Abstract

Subcritical water extraction (SWE) is an alternative technique that implement water as a solvent. The objective of this work was to compare the efficiency of SWE in temperature range from 100ºC to 180ºC at extraction times ranging from 5 to 25 minutes with an ethanolic soxhlet extraction in terms of total phenolic content (TPC), total flavonoid content (TFC) and radical scavenging activity (RSA) from Zingiber zerumbet.  Results showed the highest TPC were obtained at 180ºC and 25 min (19.88 mg GAE/g dry sample). The TFC is observed to be slightly fluctuated with time at the respective temperature. The RSA reached the maximum of 83.9 % inhibition at 180ºC and 10 minutes of SWE. A direct linear correlation shows the strong correlation were observed between TPC and RSA(R2=0.910) compared to moderate correlation (R2=0.785) perceived in TFC. It shows that using SWE, phenolic content in Zingiber zerumbet more contributed to its radical scavenging activity compared to its flavonoid content. In comparison to soxhlet extraction, SWE process for Zingiber zerumbet extract is favorable for TPC and antioxidant properties. However the values for TFC in general is lower compared to soxhlet extraction. SWE is a potential alternative extraction process that should be further explored.

Keywords


  1. Shah, M. A., Bosco, S. J. D. and Mir, S.A., “Plant extracts as natural antioxidants in meat and meat products”, Meat Science, Vol 98, (2014), 21–33
  2. Ameer, K., Shahbaz, H. M. and Kwon, J., "Green Extraction Methods for Polyphenols from Plant Matrices and Their Byproducts: A Review", Comprehensive Reviews in Food Science and Food Safety, Vol 16, (2017), 295–315.
  3. Koga, A., Beltrame, F. and Pereira, A., "Several aspects of Zingiber zerumbet: a review", Revista Brasileira de Farmacognosia, Vol 26, No 3, (2016), 1-7
  4. Alafiatayo, A. A., Ahmad, S. and Maziah, M., “Total Anti-oxidant capacity, Flavonoid, Phenolic Acid and Polyphenol Content in ten selected Species of Zingiberaceae Rhizomes”, African Journal of Traditional Complementary Alternative Medicine, Vol 11, No 3, (2014), 7–13.
  5. Prakash, R. O., Kumar, R. K., Rabinaryan, A. and Kumar, M. S.,“Pharmacognostical and Phytochemical Studies of Zingiber zerumbet (L.) SM. Rhizome”, International Journal of Research in Ayurveda & Pharmacy, Vol 2, No 3, (2011), 698–703.
  6. Nag, A., Bandyopadhyay, M. and Mukherjee, A., “Antioxidant Activities and Cytotoxicity of Zingiber zerumbet (L.) Smith Rhizome”, Journal of Pharmacognosy and Phytochemistry, Vol 2, No 3, (2013), 102–108.
  7. Ghasemzadeh, A., Jaafar, H. Z. E., Ashkani, S., Rahmat, A., Juraimi, A. S., Puteh, A. and Mohamed, M. T. M., “Variation in secondary metabolite production as well as antioxidant and antibacterial activities of Zingiber zerumbet (L.) at different stages of growth”, BMC Complementary and Alternative Medicine, Vol 16, No 104, (2016), 1-10.
  8. Rezaei, S., Najafpour, G. D., Mohammadi, M., Moghadamnia. And Kazemi, S. "Formic acid and microwave assisted extraction of curcumin from turmeric (Curcuma longa L.)”, International Journal of Engineering, Transactions B: Applications, Vol 29, No 2., (2016), 145-151.
  9. Castro-Puyana, M., Luisa-Marina, M. and Plaza, M., “Water as green extraction solvent: Principles and reasons for its use”, Current Opinion in Green and Sustainable Chemistry, Vol 5, (2017), 31-36.
  10. He, L., Zhang, X., Xu, H., Xu, C., Yuan, F., Knez, Z., Novak, Z. and Gao, Y., "Subcritical water extraction of phenolic compounds from pomegranate (Punica granatum L.) seed residues and investigation into their antioxidant activities with HPLC-ABTS + assay", Food and Bioproducts Processing, Vol 90, (2012), 215–223.
  11. Zakaria, S. M. and Kamal, S. M. M., "Subcritical Water Extraction of Bioactive Compounds from Plants and Algae: Applications in Pharmaceutical and Food Ingredients", Food Engineering Reviews, Vol 8, (2016), 23–34.
  12. Plaza, M., Amigo-Benavant, M., del Castillo, M. D., Ibáñez, E. and Herrero, M., "Neoformation of antioxidants in glycation model systems treated under subcritical water extraction conditions", Food Research International, Vol 43, (2010), 1123–1129.
  13. Ko, M. J., Lee, J. H., Nam, H. H and Chung, M. S., “Subcritical water extraction of phytochemicals from Phlomis umbrosa Turcz.” Innovative Food Science and Emerging Tehcnologies., Vol 47, (2017), 1-7.
  14. Vergara-Salinas, J. R., Pérez-Jiménez, J.., Torres, J., Agosin, E. and Pérez-Correa, J. R., “Effects of Temperature and Time on Polyphenolic Content and Antioxidant Activity in the Pressurized Hot Water Extraction of Deodorized Thyme (Thymus vulgaris). Journal of Agricultural and Food Chemistry. Vol 60, (2012), 10920–10929.
  15. Matshediso, P. G., Cukrowska, E. and Chimuka, L., "Development of pressurised hot water extraction (PHWE) for essential compounds from Moringa oleifera leaf extracts", Food Chemistry. Vol 172, (2015), 423–427.
  16. Sharifi, A., Mortazavi, S. A., Maskooki, A., Niakousari, M., Elhamirad, A. H., "Optimization of Subcritical Water Extraction of Bioactive Compounds from Barberry Fruit (Berberis vulgaris) by Using Response Surface Methodology", International Journal of Agriculture and Crop Sciences, Vol 6, 2, (2013), 89–96.
  17. Xu, H., Wang, W. and Jiang, J., Yuan, F. and Gao, Y., "Subcritical water extraction and antioxidant activity evaluation with on-line HPLC-ABTS assay of phenolic compounds from marigold (Tagetes erecta L.) flower residues", Journal of Food Science, Vol 52, 6, (2015), 3803–3811.
  18. Fan, R., Xiang, J., Li, N., Jiang, X. and Gao., Y., "Impact of extraction parameters on chemical composition and antioxidant activity of bioactive compounds from Chinese licorice (Glycyrrhiza uralensis Fisch.) by subcritical water", Separation Science and Technology, Vol 51, 4, (2016), 609–621.
  19. Rajha, H. N., Darra, N. E., Hobaika, Z., Bousette, N., Vorobiev, E., Maroun, R. G. and Louka, N., "Extraction of Total Phenolic Compounds, Flavonoids, Anthocyanins and Tannins from Grape Byproducts by Response Surface Methodology. Influence of Solid-Liquid Ratio, Particle Size , Time, Temperature and Solvent Mixtures on the Optimization Process", Food and Nutrition Sciences, Vol 5, (2014), 397–409.
  20. Khoza, B. S., Dubery, I. A., Byth-Illing, H. A., Steenkamp, P. A., Chimuka, I., and Madala, N. E., "Optimization of Pressurized Hot Water Extraction of Flavonoids from Momordica foetida Using UHPLC-qTOF-MS and Multivariate Chemometric Approaches", Food Analytical Methods, Vol 9, (2016), 1480–1489.
  21. Ko, M., Cheigh, C. I. and Chung, M. S., "Relationship analysis between flavonoids structure and subcritical water extraction (SWE)", Food chemistry, Vol 143, (2014), 147–55.
  22. Amadian-Kouchaksaraie, Z., Niazmand, R. and Najafi, M. N., "Optimization of the subcritical water extraction of phenolic antioxidants from Crocus sativus petals of saffron industry residues: Box-Behnken design and principal component analysis", Innovative Food Science & Emerging Technologies, Vol 36, (2016), 234–244.
  23. Zeković, Z., Vidović S., Vladić, J., Radosavljević, R., Cvejin, A., Elgandi, M. A. and Pavlić, B., "Optimization of subcritical water extraction of antioxidants from Coriandrum sativum seeds by response surface methodology", The Journal of Supercritical Fluid, Vol 95, (2014), 560-566.
  24. 24. Khajenoori, M. Asl, A. and Bidgoli, H., "Subcritical water extraction of essential oils from Matricaria Chamomilla L.", International Journal of Engineering, Transactions B: Applications, Vol 26, No 5., (2013), 489-494.